FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089550
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG10859.733594E-87.8986181
TATGCCG31600.06.98875594-95
GGTTTTA86400.06.77797171
GCGAATA7050.00797908756.753351
CGCGGAT9901.0556776E-46.7328851
GGCGTGA48850.06.7250091
CTCGTAT43750.06.67622694-95
CGTATGC37850.06.650341594-95
GGTTTAG38850.06.49520971
TCGTATG40000.06.470986494-95
TCTCGTA50100.06.4462294-95
GGGTTCG16254.443973E-86.44581271
GCGAATT10351.8834924E-46.44015071
GCGAGAC28850.06.4361651
GCGAAAC12851.1566208E-56.29874661
GGTCTAT26650.06.25286671
GGGGTTA32950.06.2132871
GGACTAT30000.06.1894451
GCGAAGT13101.5573656E-56.17854171
GGCGTAC9250.0024550876.17657761
Produced by FastQC (version 0.11.5)