FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089550
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT26400.07.923660894-95
GCGCGTA5500.0070289567.75145961
CGCCGTA27450.07.273472392-93
CCGTATC27150.07.26700394-95
CGTATCA22950.07.25040894-95
GCGATAA7500.0019810486.94760471
GCGTGTA15451.8066203E-86.74524741
GGTTTAC33700.06.04424761
GTGGTCG55200.05.985808494-95
TGGTCGC46300.05.9555894-95
GGTCTAA25601.4551915E-115.9212541
GGATTTT120900.05.8379941
GGATTCG17159.0678805E-75.80041171
GGTTATT49100.05.78859851
GGTTTTA80900.05.7382731
GGACTTA33250.05.69865041
GCAAATA68450.05.6747151
TCGCCGT35850.05.569227792-93
GGCTAAA49850.05.5114581
GGTCGTT13802.3345734E-45.49217751
Produced by FastQC (version 0.11.5)