FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12293828
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57800.08.29912594-95
CGTATGC69650.07.875889394-95
TCGTATG75850.07.54519194-95
TATCTCG100350.06.79161894-95
ATGCCGT64250.06.763736794-95
CTCGTAT82050.06.598894-95
ATCTCGT104900.06.315932394-95
GTATGCC84350.06.16549294-95
TCTCGTA93600.06.13971594-95
CGCCAAT152950.05.806719394-95
TCCGATC11850.00110513345.6108778
GCCGTCT65450.05.442398594-95
GCGAAAC13459.12997E-45.3102921
GCCAATA167550.05.28655994-95
ACGCCAA170800.05.241580594-95
ATATCTC146900.05.10827294-95
CCGTCTT66700.05.05558394-95
GTTCGGA15107.762137E-45.0453641
TGCCGTC71150.05.00639594-95
Produced by FastQC (version 0.11.5)