FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16448511
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA3513532.136077849235107TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT716550.4356321371581902TruSeq Adapter, Index 16 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG585150.040.4490948-49
CGTATGC597550.039.6971146-47
CCGTCTT593100.039.65666652-53
TGCCGTC594350.039.3789150-51
CTCGTAT580800.038.872244-45
ATCTCGT585800.037.12164342-43
CTTGAAA632250.035.8407162-63
CCCGATC610900.035.68172538-39
TGCTTGA657700.034.90545360-61
GTCCCGA681950.034.63554836-37
CGATCTC639200.034.19863540-41
GCCGTCT586900.033.5339752-53
ATGCCGT587650.033.50541750-51
GTCTTCT706800.033.39399754-55
TCGTATG603950.033.0065446-47
CGTCTTC602750.032.69786554-55
ACCCGTC734300.032.6155232-33
CCGTCCC734950.032.567334-35
TCTGCTT737150.032.2867558-59
GTATGCC617550.032.2335648-49
Produced by FastQC (version 0.11.5)