FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23852942
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3479531.4587424897104935Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG890300.3732453631925152Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA161300.067.897442
GATCGGG166300.066.620881
GAGCGGC185800.054.255759
CGGGAGA223200.051.523164
AGAGCGG207600.051.319078
GAGAGCG156600.047.0286067
TCGGGAG276450.041.1773723
CGCCGGA141500.037.8655746-47
CCGGATC161000.036.96272748-49
GGCGCCG234600.036.51389344-45
TCGGGGG410700.036.16901838-39
GCCGGAT165350.034.5792846-47
TCTCGGG256700.034.0093936-37
AGCGGCG160300.033.79982810-11
GGGTCGC116450.032.98570642-43
CATTAAA1371450.032.70000554-55
ATCATTA1299800.031.86981852-53
GCGCCGG129400.030.5017144-45
GTATCAT1139800.029.92677950-51
GGGCGCC339900.029.84302742-43
Produced by FastQC (version 0.11.5)