FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17759238
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1455340.8194833584639161Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG45100.023.9202181
ATCGGGA46150.023.4763682
CATTAAA574150.020.6465554-55
ATCATTA573700.019.88772252-53
CGCCGGA34450.019.17974946-47
GTATCAT551450.018.92895150-51
CCGTATC575850.018.10309248-49
TCATTAA560200.017.80891454-55
CGTATCA538900.017.64141850-51
CCGGATC41900.017.39851248-49
TCGGGGG100950.017.16181238-39
TATCATT575950.016.67718752-53
CGCCGTA630650.016.42416646-47
TTAAAAA853150.016.32782756-57
GGCGCCG59700.015.83128744-45
GCCGTAT601400.015.77382148-49
GGGTCGC36900.015.29156142-43
ATTAAAA684350.015.05920956-57
GTCGCCG716150.014.56632344-45
TAAAAAA809750.014.53670658-59
Produced by FastQC (version 0.11.5)