FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17725130
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA2322541.3103091486494034TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1520150.8576241753939182TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG542700.037.5082548-49
TGCCGTC545150.037.16958650-51
CCGTCTT549750.036.95699752-53
CGTATGC553900.036.8311546-47
CTCGTAT563150.036.0133244-45
ATCTCGT579450.034.4183642-43
TGCTTGA628200.032.0654660-61
CCGCACA641900.031.59190236-37
GTCCGCA649800.031.10095634-35
CTTGAAA644050.031.01420462-63
ACGTCCG671600.030.26337232-33
GCACATC660150.030.08482638-39
GTCTTCT693950.029.49608454-55
ACATCTC689650.028.9770640-41
TCACGTC718700.028.41072830-31
TCTGCTT728200.028.33389558-59
CTTCTGC730750.028.15370256-57
AGTCACG745450.027.63035228-29
CCAGTCA869100.023.85801726-27
TGAAAAA837750.023.72455864-65
Produced by FastQC (version 0.11.5)