FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17725130
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1388900.7835767636118889Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA40550.035.935182
GATCGGG41750.035.5692371
CGCCGTA362500.033.07760646-47
CCGTATC366550.032.9645248-49
CGTATCA357000.032.00779350-51
GTCGCCG373000.031.57693144-45
GCCGTAT365500.031.42218448-49
TCGCCGT350050.030.90911546-47
TGGTCGC371750.029.73063342-43
GTATCAT408050.029.56705750-51
GGTCGCC385550.029.1486844-45
GTGGTCG389200.027.5722742-43
ATCATTA490800.026.99680952-53
TCGGTGG455450.026.96942938-39
TCGGGGG105700.026.2768438-39
TCTCGGT464700.025.97163236-37
CGGTGGT423000.025.69984840-41
GATCTCG530800.025.52447534-35
CGGGAGA73700.025.5126064
GGCGCCG56450.025.35441444-45
Produced by FastQC (version 0.11.5)