FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854825
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT7848329.99171846603839TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA2187852.78535804425942TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT426890.5434748705413552TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTA115650.1472343432221596TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1629350.057.5178389
AGAGCAC1634700.057.3506478
CGGAAGA1662500.056.796594
TCGGAAG1670250.056.655323
ATCGGAA1681100.056.3701552
GAAGAGC1674400.056.2266356
GATCGGA1693300.056.0514451
AAGAGCA1682200.055.8784077
GGAAGAG1758550.053.6657075
TATGCCG1338850.043.5913348-49
CCGTCTT1337150.043.50345252-53
TGCCGTC1335000.043.4571150-51
CGTATGC1347000.043.41431846-47
CTCGTAT1261450.043.24822244-45
ATCTCGT1245350.042.88801642-43
AACGATC1240050.042.71077738-39
CTTGAAA1324750.042.696262-63
TGCTTGA1339600.042.62240260-61
CGATCTC1253000.042.3454340-41
GAAACGA1379650.042.17049836-37
Produced by FastQC (version 0.11.5)