FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854825
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1428811.8190220660549408Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG177660.2261794502105394Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA117250.082.934472
GATCGGG127650.077.55931
CGGGAGA157300.073.831634
TCGGGAG150900.071.6381153
GAGCGGC119100.071.182699
AGAGCGG134500.068.499648
GAGAGCG102300.066.334427
GTCGGGA40550.059.063982
GAGGGGC179650.048.6948629
GATCCGG25150.048.1688651
ATCCGGA23350.048.1052362
GTCCGGA17100.048.0442052
CCGGAGA39100.047.742684
GGAGAGC148600.046.7723056
CGGAGAG56800.046.1251265
TCCGGAG34200.045.1941263
GATCCGA13000.044.6303751
GTTCGGA13000.042.4881172
TCGGGAA35650.042.4445533
CGGGAAA45200.042.2241444
Produced by FastQC (version 0.11.5)