FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16742432
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1985011.1856162832257584TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1533310.9158227430757969TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA696250.026.75606360-61
TGCTTGA764400.024.5086358-59
TGAAAAA779150.023.75255862-63
TCTGCTT829450.022.99318556-57
CCGTCTT836050.022.50005350-51
GTCTTCT886450.021.36743752-53
CTTCTGC897300.021.23600854-55
TGCCGTC895950.020.9878348-49
TATGCCG955400.019.8061446-47
CGTATGC1041550.018.16047544-45
CTCGTAT1130650.016.57763142-43
ATCTCGT1238000.015.02889140-41
CTGCTTG747650.014.68451258-59
TTGAAAA758600.014.16107662-63
GCTTGAA775900.013.82768760-61
GTATCTC1372950.013.42713738-39
CGTCTTC814000.013.2596252-53
TTCTGCT863000.012.76854756-57
GCCGTCT860750.012.5095150-51
GAAAAAA873500.012.39083564-65
Produced by FastQC (version 0.11.5)