FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16742432
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1510020.9019119802905576Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG56500.037.8552061
ATCGGGA55800.037.1518482
CGCCGGA44950.025.94413646-47
TCGGGGG125250.025.39206738-39
GAGCGGC75350.025.034589
CCGGATC49950.024.66746948-49
CGGGAGA90300.024.1189654
AGAGCGG83900.023.3177248
GGCGCCG81150.023.26256844-45
TCTCGGG93500.021.33950636-37
GGGTCGC41750.020.55307442-43
GCCGGAT52600.019.9359246-47
GAGAGCG71100.019.8127947
CATTAAA717350.019.692454-55
ATCATTA718650.018.65047852-53
GGGGCGC79350.018.01345642-43
AGCGGCG58700.017.72358510-11
GTATCAT690000.017.12470250-51
TTAAAAA1029400.016.9747356-57
GCGCCGG44650.016.9010744-45
Produced by FastQC (version 0.11.5)