FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16499917
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2715671.6458688852798473TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2314881.402964633094821TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA869850.030.903960-61
TGAAAAA928100.028.89383562-63
TGCTTGA974100.027.66120158-59
TCTGCTT1031250.026.7708856-57
CCGTCTT1088800.025.02943850-51
CTTCTGC1112600.024.85403654-55
GTCTTCT1112550.024.53681652-53
TGCCGTC1167600.023.2995548-49
TATGCCG1254650.021.8590246-47
CGTATGC1366150.020.0620444-45
CTCGTAT1439850.018.35497742-43
ATCTCGT1560250.016.74984440-41
TTGAAAA904800.016.33483562-63
CTGCTTG948550.015.76067858-59
ATCGTAT55000.014.89605642-43
GCTTGAA987650.014.842628560-61
CAATCTC1814800.014.57841638-39
GAAAAAA1034800.014.43015864-65
TTCTGCT1067700.014.15758956-57
ACCAATC1849350.014.10963536-37
Produced by FastQC (version 0.11.5)