FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821959
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC213920.758054954023074TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG176710.6261961991651899TruSeq Adapter, Index 7 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG61700.032.55488246-47
TGCCGTC62150.031.66896248-49
CCGTCTT62600.031.59319950-51
CGTATGC63800.031.41375444-45
CTCGTAT66000.030.18673342-43
ATCTCGT68800.028.64731240-41
TGCTTGA75400.025.79352258-59
CTTGAAA76950.024.8419360-61
GTCTTCT83900.023.53208252-53
GATCATC84550.023.25450536-37
TCTGCTT90650.022.4237356-57
CTTCTGC91950.022.106954-55
CAGATCA95850.021.15766134-35
ACCAGAT97900.020.83701532-33
TCATCTC96000.020.80248338-39
AGTCACC102900.019.97839728-29
TGAAAAA99050.019.4428862-63
CCAGTCA107700.018.95559326-27
TCACCAG109300.018.62735430-31
GCCGTCT61750.017.90031248-49
Produced by FastQC (version 0.11.5)