FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821959
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC105700.37456249364360006Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG7350.035.3583371
ATCGGGA7700.033.751142
TCGGGGG16400.025.40589538-39
CGCCGTA37200.024.46534746-47
CCGTATC37800.024.20339448-49
CGGGAGA13250.023.8240184
GTCGCCG39200.022.8553644-45
CGTATCA37150.022.56297750-51
GAGCGGC9950.022.4601219
GCCGTAT37350.022.43816848-49
AGAGCGG10800.021.5542288
TCGCCGT37000.021.32630346-47
GAGAGCG9550.020.949497
GGCGCCG8600.020.83558544-45
TCGGGAG13950.020.6256963
GTATCAT45250.020.32503150-51
TGGTCGC40800.020.02338842-43
ATCATTA54200.019.37964452-53
GGTCGCC42400.019.2094844-45
CGCCGGA6000.018.62119746-47
Produced by FastQC (version 0.11.5)