FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956345
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC270630.9154208997934951TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG92950.31440850103759876TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57200.033.74802446-47
CCGTCTT58500.032.6328850-51
CGTATGC59300.032.39423444-45
TGCCGTC59950.031.8039448-49
CTCGTAT60000.031.58097542-43
ATCTCGT63050.029.94028740-41
TAATCTC69700.026.87931338-39
TGCTTGA70100.026.76116658-59
ATGCCGT58300.025.5266746-47
GCCGTCT58550.025.49931348-49
TCGTATG60100.025.04890342-43
CTTGAAA75250.024.70835760-61
CGTCTTC62150.024.29004550-51
TCTCGTA61700.023.89903640-41
GTATGCC63650.023.72644244-45
CTTGTAA81150.023.58615334-35
GTCTTCT82700.023.52972252-53
ATCGGAA131500.023.4817852
TCGGAAG131600.023.4575923
CGGAAGA131300.023.4388474
Produced by FastQC (version 0.11.5)