FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956345
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC59770.20217532121589327Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA7300.041.9557152
GATCGGG8050.039.1997341
AGAGCGG13250.029.1732568
GAGCGGC13900.026.1346849
CGGGAGA15450.024.3418944
CCGTATC26050.023.23132948-49
CGCCGTA25400.022.99777446-47
TCGGGAG15050.022.5090123
GGCGCCG9200.022.50860444-45
TCGGGGG19650.021.79441838-39
AGCGGCG9350.020.41924510-11
GTCGCCG27500.020.05219844-45
GAGAGCG10700.019.1370377
CGCCGGA5050.018.89462746-47
CGTATCA25500.018.71478350-51
GCCGGAT5800.018.45761946-47
GCCGTAT25950.018.38759648-49
GGGCGCC14200.018.3566742-43
GTATCAT32750.018.2680850-51
TCGCCGT25350.017.90203946-47
Produced by FastQC (version 0.11.5)