FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9535677
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC310260.3253675643585663Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA61350.032.2354846-47
CCGTATC63400.031.57012448-49
CGTATCA60200.030.9297450-51
GCCGTAT61400.030.43296848-49
GTCGCCG69650.029.44896944-45
TCGCCGT62900.028.57254646-47
TGGTCGC71200.027.10605642-43
GGTCGCC73500.025.77466444-45
GTGGTCG76000.024.0822242-43
GTATCAT87400.022.93441850-51
GATCTCG101900.022.3343634-35
TCGGTGG94600.022.22851838-39
TCTCGGT94150.021.9803436-37
AGAGCGT188850.020.9843039
CGGTGGT89600.020.53108240-41
AAGAGCG202600.019.6300938
TAGATCT115450.018.90824132-33
ATCTCGG106500.018.85206836-37
CTCGGTG103150.018.82140238-39
ATCATTA120050.018.7958852-53
Produced by FastQC (version 0.11.5)