FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11360757
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA188320.16576360184448977TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT159950.14079167435761544TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59650.032.79764648-49
CGTATGC63700.030.86166646-47
CTCGTAT60750.030.0354344-45
CCCGATC67900.026.05392338-39
ATCTCGT68600.025.82268142-43
TGCCGTC77900.025.05295650-51
GTCCCGA80050.024.53193336-37
CCGTCTT80400.024.24803752-53
ACCCGTC84100.023.7195432-33
CGATCTC84300.021.46416940-41
CCGTCCC98550.020.38536834-35
TCACCCG106600.018.8265730-31
TCGTATG63900.017.7447946-47
TGCTTGA123050.016.1530160-61
ATGCCGT69350.016.11033850-51
TCTCGTA64150.015.74023444-45
CCGATCT65550.015.32337140-41
CGTCCCG74400.015.12840136-37
CGTCTGA138200.014.81160516-17
TCCCGAT73000.014.50770238-39
Produced by FastQC (version 0.11.5)