FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7591918
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC238140.31367567457920387Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA78050.017.82862950-51
CCGTATC84800.017.63687948-49
CGCCGTA89300.016.6138346-47
GCCGTAT86300.016.36753348-49
GTATCAT93250.016.14483650-51
TCGCCGT92750.014.53702846-47
CATTAAA123650.014.42754854-55
ATCATTA117450.014.23612552-53
GTCGCCG103900.014.23262644-45
TCATTAA118050.012.93873254-55
GGTCGCC111400.012.65650544-45
TATCATT115500.012.48184652-53
TGGTCGC112400.012.37524642-43
GTGGTCG121850.011.08432742-43
TCGGTGG153950.010.3435138-39
CGGTGGT143900.09.63252640-41
ATTAAAA172300.09.50144756-57
TCTCGGT163900.09.35356636-37
TTAAAAA247200.09.34272656-57
TCGGGGG35050.09.14051938-39
Produced by FastQC (version 0.11.5)