FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3818332
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA739561.9368666737203575TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT240670.6303013986211781TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTA48290.12646883508296294TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG146200.039.4135448-49
CCGTCTT144700.039.31985552-53
TGCCGTC146450.038.85930350-51
CGTATGC148550.038.77403346-47
CTCGTAT141850.037.58550344-45
CTTGAAA152050.036.85886462-63
ATCTCGT145400.035.58659742-43
TGCTTGA162300.034.57359360-61
AACGATC150550.034.27461238-39
GTCTTCT169300.033.57890354-55
TCTGCTT175650.032.75703458-59
GAAACGA177800.032.2269536-37
CGATCTC161300.032.09340340-41
TGAAAAA174000.031.86794364-65
CTTCTGC183150.031.2989456-57
GCCGTCT144600.030.31496252-53
ATGCCGT145250.030.2281150-51
CGTCTTC146300.030.01179154-55
ACGTGAA193550.029.81424332-33
ATCGTAT11850.029.673644-45
Produced by FastQC (version 0.11.5)