FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3818332
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC703891.8434489195805919Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA107850.038.16190350-51
CCGTATC111750.037.66581748-49
CGCCGTA113950.036.9756746-47
GCCGTAT112850.036.7306748-49
GTATCAT115500.036.33341250-51
TCGCCGT111950.035.40987846-47
GTCGCCG120600.035.1310944-45
ATCATTA131800.034.4787152-53
CATTAAA138500.033.9336654-55
GGTCGCC124800.033.54939344-45
TCATTAA131050.033.4694754-55
TGGTCGC120200.033.2733242-43
TATCATT125700.032.8258552-53
GTGGTCG124650.032.0473842-43
TCGGTGG144350.029.9377938-39
CGGTGGT134500.029.84100540-41
TCTCGGT147050.028.8866736-37
CTCGGTG147450.028.45535938-39
GGTGGTC143950.028.07987240-41
GATCTCG167400.027.67066234-35
Produced by FastQC (version 0.11.5)