FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3704084
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA292820.7905328280892119TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT200390.5409974503817948TruSeq Adapter, Index 14 (97% over 44bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT79350.032.45674552-53
TATGCCG80550.032.34507848-49
TGCCGTC80900.031.70561250-51
CGTATGC84500.030.86122346-47
CTCGTAT88200.029.48001744-45
TGCTTGA91550.027.468160-61
ATCTCGT95150.027.2265342-43
CTTGAAA95350.026.42268262-63
CCGTATC97600.025.27796238-39
GTATCTC104500.023.81339640-41
TTCCGTA105350.023.68935836-37
GTCTTCT110350.023.32625454-55
TCTGCTT112550.023.22927958-59
AGTTCCG112550.022.93560634-35
CTTCTGC117850.021.96295756-57
TGAAAAA128250.019.57093464-65
GCCGTCT79200.019.1689552-53
ACAGTTC136750.019.13858232-33
ATGCCGT80800.018.85861850-51
CGTCTTC82250.018.44815854-55
Produced by FastQC (version 0.11.5)