FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360777
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA21680.15932074101781554TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT16740.1230179522434609TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8650.023.67088148-49
CGTATGC9300.021.50445446-47
CTCGTAT9750.020.7271144-45
TGCCGTC10300.019.41664350-51
CCGTCTT11000.018.34778852-53
ATCTCGT11900.017.35824842-43
ATGCCGT9150.015.09172850-51
TCGTATG9300.014.33630246-47
GCCGTCT9750.014.12475552-53
GAATCTC16400.012.8848640-41
CGTCTTC10950.012.36203854-55
TCTCGTA10650.012.27827944-45
TGCTTGA16050.011.98437560-61
GTATGCC11850.011.4521948-49
ACATGTC19200.011.37721932-33
GTCAGAA19600.011.2657636-37
AATCTCG11600.011.25740542-43
CGTCTGA23350.010.98172216-17
CCAGTCA23900.010.928493526-27
ATGTCAG19850.010.765038534-35
Produced by FastQC (version 0.11.5)