FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360777
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC22640.16637553397801402Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA6250.020.51129546-47
CGTATCA6150.020.4674850-51
CCGTATC6800.019.55305348-49
GCCGTAT6550.018.4869148-49
TCGCCGT6350.018.31899846-47
GTCGCCG7050.018.18176344-45
GGTCGCC8000.015.42924644-45
TGGTCGC7700.015.4129842-43
CCGAGCG1950.004489416714.6019169
TCGGTGG11300.014.28097238-39
GTGGTCG9050.012.85152942-43
GTATCAT10850.012.69585350-51
CGGTGGT10100.012.21960840-41
TCTCGGT11450.012.01903836-37
GATCTCG12700.011.95593334-35
ATCTCGG12600.010.73375136-37
CTCGGTG12700.010.27747138-39
TAGATCT14800.09.93905632-33
GGCGCCG2951.4030837E-59.65585144-45
AGAGCGT26150.09.6182799
Produced by FastQC (version 0.11.5)