FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471277
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3453.6379788E-1213.11425948-49
CTCGTAT3801.8189894E-1212.51467544-45
CGTATGC4050.012.347342546-47
TCGTATG4109.268515E-78.71197546-47
TGCCGTC6101.2259989E-98.19782550-51
ATCTCGT4801.0787589E-67.914378642-43
ACCCGTC6606.002665E-97.55514732-33
ATGCCGT4107.618914E-57.550378350-51
CCCGATC5702.3297616E-77.497832338-39
GTCCCGA6708.034476E-97.442004736-37
CCGTCTT6201.2229066E-77.276245652-53
TCTCGTA4251.21030505E-47.27323544-45
CGATCTC6703.377534E-66.37875340-41
TCACCCG9151.15833245E-86.22834230-31
GCCGTCT5504.2739883E-46.0438152-53
GAAGTAA9700.0042890495.874793
CCGTCCC8953.1004856E-75.83649934-35
GATCTCG6505.9932616E-45.47918542-43
ACGTCTG14605.2386895E-105.204328594-95
CGTCTTC7050.00176945925.052245654-55
Produced by FastQC (version 0.11.5)