FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13917364
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30750.024.76993446-47
CGTATGC37550.020.32236544-45
TGCCGTC37150.020.2464248-49
CTCGTAT37600.019.891342-43
CCGTCTT46800.016.22425750-51
ATCTCGT47900.015.61404940-41
GCCGTCT37650.013.65553648-49
TCTCGTA38350.013.31108940-41
ATGCCGT38400.013.26485546-47
TCGTATG42050.012.59155542-43
CGTCTTC47300.010.869575550-51
CATCTCG54150.09.86562438-39
GTATGCC55950.09.68754944-45
GCGTCGA2050.0055435729.26739764-65
GATCATC90350.08.40943836-37
CGTCTGA105100.07.52327616-17
ACCAGAT108550.07.503098532-33
AGTCACC117400.06.958116528-29
CCAGTCA121400.06.865679726-27
CACGTCT121350.06.61364414-15
Produced by FastQC (version 0.11.5)