FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14903416
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG164520.11039079899534442TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60800.021.61323446-47
TGCCGTC68100.019.15656948-49
CGTATGC69350.018.820644-45
CCGTCTT72850.018.20161450-51
CTCGTAT70900.017.8489542-43
ATCTCGT86500.014.52015540-41
GCCGTCT65750.014.08439350-51
ATGCCGT66800.013.82736948-49
CGTCTTC72350.012.96343552-53
TCGTATG74200.012.65612644-45
TCTCGTA77250.011.80223942-43
GTATGCC88800.010.77698746-47
CTTGAAA155100.09.2328360-61
TAATCTC167750.08.09590138-39
GTCTTCT181250.07.794720652-53
AATCTCG120250.07.77934140-41
CTTGTAA193350.07.34329334-35
ACCTTGT193950.07.29637432-33
TCTGCTT199550.07.25901356-57
AGTCACC189750.07.12040128-29
Produced by FastQC (version 0.11.5)