FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754589
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC42960.5693165418525847Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA2750.049.877752
GATCGGG3050.046.522491
CGGGAGA4750.034.871864
AGAGCGG4450.030.923758
GTCGGGA1901.2732926E-1129.8723372
GAGCGGC4200.029.3769789
GAGAGCG3850.025.8777267
TCGGGGG8050.025.6400438-39
TTTCGGG1800.023.71967336-37
GTCCGGA1000.001694663323.6489332
TCGGGAG7350.023.1663023
GGCGCCG3600.023.07306344-45
GCCGGAT2800.022.89003846-47
CGCCGGA2350.022.22263746-47
CCGGATC2700.021.98604248-49
GGGTCGC2800.021.18602442-43
CGTCGGG2250.021.10098512-13
GGTCGGG3151.4551915E-1121.0212751
CATTAAA22850.020.58437554-55
ATTTCGG1501.6916601E-1020.56046734-35
Produced by FastQC (version 0.11.5)