FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12616945
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA358060.2837929467077807TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT179150.1419915835410236TruSeq Adapter, Index 13 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT129600.024.0744752-53
TGCCGTC129900.023.8373150-51
TATGCCG137050.022.87156348-49
CGTATGC158600.019.86890446-47
CTCGTAT165850.018.11757344-45
CTTGAAA176500.018.01613662-63
CGTCTTC119400.017.30205354-55
GCCGTCT123800.016.6289552-53
ATCTCGT189500.015.67459842-43
TCTGCTT205700.015.56133858-59
GTCTTCT204950.015.38625454-55
ATGCCGT136000.015.29542850-51
CTTCTGC244700.013.44005956-57
GTATGCC161900.012.92204148-49
CTGCTTG164200.012.90046760-61
TCGTATG167900.012.53114146-47
TGCTTGA264750.012.15393960-61
TGAAAAA275200.011.761970564-65
AACAATC266700.011.306499538-39
TCTCGTA175050.011.27156944-45
Produced by FastQC (version 0.11.5)