FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12616945
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC326120.258477785232479Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA103850.018.22856550-51
CGCCGGA9450.017.33526646-47
CCGTATC117050.017.2634848-49
CGCCGTA121200.016.49387446-47
GCCGTAT116150.016.3546448-49
GTATCAT127200.016.0587650-51
CCGGATC11400.015.20506348-49
GTCGCCG141250.014.38504744-45
TCGCCGT126800.014.15519146-47
GATCGGG17100.014.111751
TCATTAA150650.013.87953654-55
ATCATTA168550.013.85511452-53
CATTAAA181250.013.45026254-55
ATCGGGA18150.012.7739812
GGTCGCC152850.012.56345444-45
TGGTCGC158800.012.1242342-43
GGCGCCG18500.011.29111844-45
TATCATT175350.011.16366652-53
GTGGTCG172000.010.69687842-43
TCGGGGG38250.09.80524738-39
Produced by FastQC (version 0.11.5)