FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11152879
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT59000.010.86609952-53
TGCCGTC58150.010.72522150-51
TATGCCG60050.010.38587148-49
CGTATGC74500.08.5311946-47
CTCGTAT84200.07.51045544-45
ATGCCGT58500.07.34661994-95
TATCTCG108150.06.95981394-95
CTTGAAA108900.06.956143462-63
TCTCGTA92150.06.87987894-95
CGTCTTC54000.06.771759554-55
ATCTCGT106000.06.720169594-95
CCGTATC140850.06.658939494-95
TCCGTAT145100.06.59481394-95
GTATGCC82050.06.453437394-95
TCGTATG81300.06.42535394-95
CGTATCT135300.06.352952594-95
GCCGTCT54000.06.331850552-53
TTCCGTA159750.05.975099692-93
AGTTCCG177700.05.79918794-95
GTCTTCT123100.05.767501454-55
Produced by FastQC (version 0.11.5)