FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11152879
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT55850.08.67775294-95
CGCCGTA58400.08.29884394-95
CGTATCA47900.08.08448494-95
TGGTCGC87450.07.769739694-95
TCGCCGT63400.07.381438792-93
GGTCGCC84200.07.33603694-95
GTGGTCG97900.07.304391494-95
CCGTATC60300.07.288776494-95
GTCGCCG72000.07.259227394-95
TCGGTGG138050.07.1246694-95
ATATGCG10553.604842E-56.72832541
CCGGATC7456.145274E-86.693588348-49
CTCGGTG149050.06.5032294-95
CGGTGGT134250.06.19375494-95
TCTCGGT154500.06.07341192-93
GTATCAT64250.05.87807750-51
GGTGGTC137400.05.481163594-95
TAGATCT215650.05.32105794-95
CGCCGGA6800.00109809585.23743646-47
AGTGTAG265050.05.22565894-95
Produced by FastQC (version 0.11.5)