FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20968482
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1207530.5758785972203424Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA59800.042.449462
GATCGGG62800.041.7517621
CGCCGTA310000.034.50501646-47
CCGTATC316150.034.11007348-49
CGTATCA302600.032.60530550-51
GTCGCCG330200.032.15984344-45
GCCGTAT309850.031.9408848-49
TCGCCGT302800.031.01261946-47
GTATCAT351800.030.52094750-51
TGGTCGC326450.030.17952242-43
GGTCGCC347600.028.1598344-45
ATCATTA438350.027.94188552-53
GTGGTCG349150.026.77052342-43
TCGGTGG420300.026.39309338-39
CATTAAA502600.025.8959854-55
CGGGAGA118250.025.5598664
TCTCGGT425750.025.28299736-37
TCGGGGG151750.025.18012238-39
GATCTCG515050.024.35425834-35
CGGTGGT387700.024.33863840-41
Produced by FastQC (version 0.11.5)