FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18856353
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC321740.170626843907727Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG202600.10744389437342416Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA139200.069.336472
GATCGGG144800.067.872691
CGGGAGA199400.056.1307564
AGAGCGG167300.052.826178
GAGCGGC157800.052.2920469
TCGGGAG208700.051.1156853
GAGAGCG143450.048.238997
GTCGGGA59350.038.779182
CGCCGGA96900.036.82316646-47
GCCGGAT123100.035.75495546-47
CCGGATC114950.035.2376248-49
TCGGGGG354550.035.2228438-39
GGCGCCG166850.033.69531644-45
TCTCGGG172650.032.19397736-37
AGCGGCG141400.032.0555810-11
CGGATCA123250.030.9758548-49
CGGGTAG77400.029.78816414-15
TAGGGGA170550.029.74031318-19
CCGTATC227550.029.1359748-49
GGGCGCC248100.028.92541142-43
Produced by FastQC (version 0.11.5)