FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18302211
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1109100.6059923579724876TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA641350.3504221429858939TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG249700.038.89278448-49
CGTATGC255200.038.07320446-47
CTCGTAT245800.037.2150444-45
TGCCGTC268700.035.930350-51
CCGTCTT269700.035.77287752-53
ATCTCGT257800.035.1027442-43
AGTCAAC343000.028.49534834-35
AACAATC340250.026.91063138-39
TGCTTGA357900.026.87242360-61
CAATCTC344650.026.64975540-41
CGTCTGA397850.024.93669316-17
TCGTATG258550.024.2420844-45
ATGCCGT260950.024.0332848-49
GCCGTCT261900.023.86440550-51
CTTGAAA405250.023.85671262-63
CACGTCT419700.023.6610314-15
ATCGGAA540300.023.6605852
TCTCGTA249100.023.6599142-43
GATCGGA542750.023.6335771
TCGGAAG543500.023.571663
Produced by FastQC (version 0.11.5)