FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18302211
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC584480.3193493944529434Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG38400.033.674921
CGCCGTA121500.032.8836946-47
GTCGCCG133300.030.9319544-45
ATCGGGA41050.030.9236552
CCGTATC130400.030.73351348-49
TGGTCGC133050.027.99222442-43
TCGCCGT119950.027.07438546-47
CGTATCA125800.026.82510450-51
GCCGTAT126150.026.68740348-49
GATCTCG202450.024.75780534-35
TCGGTGG177650.024.52936238-39
TCTCGGT175000.023.80123136-37
GGTCGCC144950.023.5820744-45
GTGGTCG143650.022.95228642-43
GTATCAT187850.021.44088250-51
TCGGGGG93000.020.77400438-39
CGGTGGT162400.020.38928840-41
AGAGCGG64250.019.966818
AGAGCGT358150.019.7274079
CGGGAGA73500.019.6837864
Produced by FastQC (version 0.11.5)