FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17194358
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT930420.5411193601994329TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA801820.4663273848316989TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG259800.035.8217748-49
CGTATGC265200.035.11925546-47
CTCGTAT267700.034.4973844-45
CCGTCTT279550.032.9326752-53
TGCCGTC280200.032.7810950-51
ATCTCGT280200.032.4915842-43
CCGTATC276900.032.4843338-39
TTCCGTA294400.031.07764236-37
AGTTCCG309350.030.34410334-35
GTATCTC321400.028.18613440-41
TGCTTGA351700.025.7866660-61
CTTGAAA380600.024.11054262-63
ACAGTTC402950.023.57319332-33
CGTCTGA433750.021.94754616-17
GTCTTCT424500.021.8850354-55
CCAGTCA443200.021.48744826-27
AGTCACA450500.021.19192928-29
TCACAGT458500.020.7558930-31
CACGTCT460350.020.72075714-15
TCTGCTT459100.020.49947758-59
Produced by FastQC (version 0.11.5)