FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17194358
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC709820.41282146155151594Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA149800.029.39745546-47
GATCGGG35000.029.3672331
ATCGGGA35550.028.380262
CCGTATC156750.028.30911448-49
GTCGCCG159800.027.9564844-45
CGTATCA150000.026.80451650-51
GCCGTAT152750.026.48578648-49
TCGCCGT149200.026.04698846-47
TGGTCGC163200.025.48284542-43
GGTCGCC172550.023.7171244-45
TCGGTGG211450.022.57133938-39
GATCTCG235650.022.49839834-35
GTGGTCG175500.022.46603842-43
TCTCGGT204800.022.38830836-37
GTATCAT201850.022.01340950-51
CGGTGGT194600.020.47930740-41
AGAGCGT420950.020.2282839
ATCATTA267500.019.42810252-53
TAGATCT260950.019.18728632-33
AAGAGCG455650.018.8229838
Produced by FastQC (version 0.11.5)