FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10540108
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT486530.46159868570606677TruSeq Adapter, Index 20 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA279380.26506369763953086TruSeq Adapter, Index 20 (97% over 44bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT149300.027.38640852-53
TATGCCG150350.027.30872748-49
TGCCGTC151150.027.00671650-51
CGTATGC162400.025.19449446-47
CTCGTAT174500.023.26867344-45
CTTGAAA184950.022.1983662-63
TGCTTGA195550.021.27476760-61
ATCTCGT199850.020.04288742-43
GCCGTCT146650.018.08085350-51
ATGCCGT151450.017.61781948-49
GTCTTCT239150.017.49573754-55
TCTGCTT244350.017.22059258-59
CGTCTTC155100.017.1615152-53
TGAAAAA248500.016.49281164-65
TTATCTC258600.015.75571540-41
CTTCTGC268650.015.70714756-57
TCGTATG169050.015.69380244-45
GTATGCC171550.015.62296546-47
CCTTATC265700.015.21852138-39
GGCCTTA277300.014.7703836-37
Produced by FastQC (version 0.11.5)