FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10540108
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC237110.2249597442455049Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG23700.021.9656411
ATCGGGA24250.021.0771332
CCGTATC96950.016.9502448-49
CGCCGTA99350.016.44289246-47
CGTATCA86150.015.90879950-51
GTATCAT109550.014.939074550-51
ATCATTA136050.014.47671652-53
GGCGCCG30400.014.44832644-45
CGCCGGA15550.014.3534146-47
GCCGTAT96750.014.21167248-49
TCGGGGG52100.014.16854538-39
AGAGCGG43050.014.1087868
GTCGCCG116200.013.79167644-45
CATTAAA160800.013.60931654-55
GAGCGGC40700.013.2915519
CCGGATC19500.012.90888948-49
CGGGAGA54900.012.6774554
TCGCCGT103700.012.45597646-47
GCCGGAT18450.011.58253946-47
TGGTCGC131750.011.18939542-43
Produced by FastQC (version 0.11.5)