FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22510182
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71650.012.52402748-49
CGTATGC80100.011.41083946-47
CTCGTAT87500.010.3513644-45
TGCCGTC96250.09.71875550-51
CCGTCTT100050.09.63533552-53
ATCTCGT113750.08.05720842-43
TCGTATG85550.07.31736346-47
ATGCCGT88450.07.15818150-51
GCCGTCT92850.06.776755352-53
CGGAATC140050.06.51023738-39
TCTCGTA99500.06.188098444-45
CGTTTCG162550.05.945243494-95
TTCGGAA157550.05.922755236-37
GTATGCC110150.05.877643648-49
CGTCTTC121000.05.69123754-55
GTTTCGG168150.05.676576634-35
TCGGAAT139600.05.39182894-95
TTTCGGA169250.05.373191494-95
AGTCACG248500.05.283977594-95
AATCTCG132500.05.26841742-43
Produced by FastQC (version 0.11.5)