FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7577305
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC122500.1616669779030935Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA25250.031.48048846-47
CCGTATC29350.027.64723848-49
CGTATCA26300.026.29688350-51
GCCGTAT26450.025.96557248-49
GTCGCCG29850.025.91578544-45
TCGCCGT27000.024.21403346-47
TGGTCGC30050.024.17819842-43
GATCGGG14150.022.2015781
GGTCGCC32850.020.68594244-45
ATCGGGA15400.019.4860922
GTGGTCG35600.018.559542-43
TCTCGGT43200.018.39244336-37
TCGGTGG47600.018.32305338-39
GATCTCG52700.017.93201434-35
GTATCAT46700.017.5297250-51
CGGTGGT44700.014.98968940-41
TAGATCT64600.013.68242932-33
ATCTCGG53600.013.55189936-37
ATCATTA72600.013.54735252-53
CTCGGTG52550.013.15241438-39
Produced by FastQC (version 0.11.5)