FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6453169
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG259450.4020505274230382TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC167720.2599033126205125TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC60100.038.8036144-45
TATGCCG60100.038.68746646-47
CTCGTAT59500.038.2346242-43
ATCTCGT66400.033.9395740-41
TGCCGTC69100.033.13302248-49
CCGTCTT69600.032.89499750-51
TAATCTC81800.027.84027138-39
CTTGTAA98900.024.1552534-35
TCGTATG61150.023.84553344-45
TGCTTGA98900.022.79164758-59
TCTCGTA61900.022.78788642-43
ATGCCGT64100.022.63837448-49
CTTGAAA102900.021.85900160-61
CGTCTGA110400.021.63916416-17
AATCTCG66700.021.39719640-41
ACCTTGT112450.021.28748932-33
GCCGTCT67350.021.2285850-51
AGTCACC113650.021.1299128-29
GTATGCC70350.020.96474346-47
CCAGTCA118500.020.1849226-27
Produced by FastQC (version 0.11.5)