FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6453169
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC218390.3384228740948827Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA36600.037.78887646-47
GCCGTAT36850.036.2606848-49
CGTATCA37700.035.8225550-51
CCGTATC41400.034.03702548-49
TCGCCGT38750.032.83925646-47
GTCGCCG42700.032.11016544-45
TGGTCGC41300.031.77293642-43
GGTCGCC44650.029.7597244-45
GTGGTCG47150.027.18241342-43
TCTCGGT55900.025.82339336-37
TCGGTGG58250.025.46891438-39
GATCTCG62950.025.0977834-35
GTATCAT56650.024.96091350-51
AGAGCGT112150.024.748989
CGGTGGT55950.023.36605840-41
TAGATCT66600.022.76784532-33
AAGAGCG124600.022.613638
ATCTCGG64400.022.2324936-37
CTCGGTG63350.022.0453438-39
ATCATTA71650.021.61243652-53
Produced by FastQC (version 0.11.5)