FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24327604
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA1027130.42220762883184054TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT590640.24278593156975098TruSeq Adapter, Index 27 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA480350.017.54891262-63
CCGTCTT587900.014.4346952-53
TGAAAAA590900.014.3179764-65
TGCTTGA626050.014.003592560-61
TCTGCTT641750.013.67970258-59
TGCCGTC656250.012.87006650-51
GTCTTCT696150.012.48450554-55
CTTCTGC738300.011.95508656-57
TATGCCG737400.011.52152348-49
CTGCTTG536800.010.62987260-61
TTGAAAA545000.010.25627364-65
CGTATGC859350.09.90035946-47
CGTCTTC563300.09.85171854-55
GCCGTCT611050.09.03835552-53
TTCTGCT670500.08.6661958-59
CTCGTAT1000900.08.41790244-45
GCTTGAA708900.08.34053762-63
GAAAAAA716200.08.0035466-67
ATGCCGT697800.07.941791550-51
TCTTCTG828000.07.227085656-57
Produced by FastQC (version 0.11.5)