FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24327604
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC873850.3592010129727531Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA571150.011.02856754-55
ATCGGGA62850.010.4648942
GATCGGG63900.010.4410561
ATCATTA579950.010.32744752-53
TCGGGGG106950.09.94145838-39
CGCCGGA38000.09.87260846-47
TCATTAA571800.09.71538354-55
TTAAAAA846750.09.34316356-57
GTATCAT596500.09.1866950-51
GGCGCCG70300.09.08484944-45
CCGGATC49100.08.89898448-49
CGTATCA593050.08.73146550-51
TATCATT606250.08.7116152-53
TAAAAAA810350.08.70135258-59
ATTAAAA683300.08.59054856-57
CCGTATC647350.08.40774648-49
GGGTCGC42300.07.688259642-43
GCCGTAT696200.07.452835648-49
GGTCGCC983850.07.397512494-95
GTCGCCG895900.07.320102794-95
Produced by FastQC (version 0.11.5)