FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367985
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG765420.6188720312969331TruSeq Adapter, Index 1 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC530180.4286712831556636TruSeq Adapter, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT230200.032.40889750-51
TATGCCG234450.032.2578746-47
TGCCGTC235650.031.5785748-49
CGTATGC251400.030.07772844-45
CTCGTAT252900.027.69714242-43
TGCTTGA269600.027.64812558-59
CTTGAAA274350.027.08353860-61
ATCTCGT277400.024.84862540-41
GTCTTCT328350.022.99672152-53
TCTGCTT345050.022.33164856-57
CGATCTC321700.021.54491838-39
CTTCTGC365750.020.95739654-55
CACGATC328900.020.95054636-37
TGAAAAA357100.020.90075562-63
GCCGTCT227550.019.45217350-51
ATCACGA389950.019.42433534-35
ATGCCGT233000.019.0993148-49
CGTCTTC239350.018.60131652-53
GTATGCC262200.017.21736346-47
TCGTATG261650.017.21611244-45
Produced by FastQC (version 0.11.5)