FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367985
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC753920.6095738311454938Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC153550.029.76985548-49
CGTATCA146250.029.38163850-51
CGCCGTA158050.028.9173246-47
GCCGTAT155650.027.73579248-49
GTATCAT178100.025.8077650-51
GTCGCCG181350.025.49814844-45
TCGCCGT162250.025.4177446-47
ATCATTA212700.024.10544252-53
TGGTCGC194250.022.5123342-43
CATTAAA242350.022.3662354-55
GGTCGCC196000.022.33274544-45
GATCGGG27350.020.7630941
TATCATT209900.020.73691652-53
TCATTAA224800.020.64507354-55
GTGGTCG211350.019.94952442-43
ATCGGGA27900.019.844942
TCGGTGG271450.017.82931538-39
CGGTGGT247550.017.28991740-41
TCTCGGT289500.016.29814136-37
GGTGGTC275950.016.21590240-41
Produced by FastQC (version 0.11.5)