FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15978068
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC800990.5013059150831002Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA56200.044.0375862
GATCGGG57650.043.668721
AGAGCGG99000.029.339218
GAGCGGC90000.029.1097419
CGGGAGA103500.028.9424344
CCGTATC213150.028.72994648-49
CGCCGTA216900.027.86816246-47
CGCCGGA45050.026.98265346-47
TCGGGGG140650.026.47931938-39
GTATCAT231600.026.33610250-51
ATCATTA296200.026.04239752-53
CCGGATC55450.025.47910948-49
GGCGCCG75900.025.08303344-45
GTCGCCG248400.025.03785544-45
CATTAAA349150.024.9616954-55
GAGAGCG77850.024.566347
CGTATCA196250.023.95141450-51
GCCGGAT53250.023.40718346-47
TCGGGAG121250.023.2607653
GCCGTAT215450.021.9649248-49
Produced by FastQC (version 0.11.5)